The call to depart from Babylon is the culmination of the multitudinous and resounding call of Scripture that God’s people be separate, be set apart from this world, and in some cases, actually leave the place of contaminants. In this study, we examine what Babylon is and what it represents and why our need for separation is reflected in the final call of God for the tribulation saints to leave Babylon. The implications of this call for us are these: 1) See the world for what it is, a harlot; 2) See the Devil’s ploy behind the harlot; 3) Delight yourself in the Lord; 4) Run from Babylon and run toward Jerusalem.